Consider a protein composed of only 21 amino acids encoded by a short ORF that is located between two known genes in a mammalian genome. Due to its length, the ORF had not been annotated as a gene. Which of the following methods can demonstrate that it is indeed a gene that is actively translated to protein?
A) RNA-seq
B) RT-PCR
C) DNA microarray analysis
D) ChIP-seq
E) Ribosome profiling

Question

Quizplus · Accepted Answer

As revealed by ribosome profiling, many open reading frames (ORFs) that are too short to be normally annotated as genes are actively translated and probably encode functional, albeit very small, proteins.

Consider a Protein Composed of Only 21 Amino Acids Encoded