Question 1

How is sigma H degradation controlled by temperature, and why is this important to the cell?

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Question 2

During the process of endospore formation, different sigma factors are active in the mother cell and forespore. How is this possible, and why is it important?

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Question 3

Explain the process of bioluminescence in Allivibrio fischeri. How is this an example of a mutualistic relationship?

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Question 4

What does the gene inversion in Salmonella enterica accomplish with respect to its course of infection? Briefly describe how this mechanism works.

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Question 5

Explain what happens to motility when a chemoattractant is added to bacterial cells.

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Question 6

Explain how small, untranslated RNAs (sRNAs) regulate iron uptake and iron storage.

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Question 7

Briefly explain two-dimensional gel electrophoresis. What is the procedure used to determine, and why are both dimensions necessary?

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Question 8

Explain how riboswitches can be compared to activator and repressor proteins. State an example.

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Question 9

The stringent response downregulates rRNA synthesis. Does it similarly downregulate ribosomal protein synthesis?

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Question 10

Explain the process of attenuation in regulating transcription of the tryptophan operon.

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Question 11

Quorum sensing plays a role in interspecies communications. Describe the relationship between green seaweed Enteromorpha and Vibrio anguillarum bacterial cells in biofilms, and explain how Escherichia coli was used to understand this interaction.

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Question 12

Explain how a repressor protein works with respect to a biosynthetic operon.

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Question 13

What happens when strand slippage occurs on the template or the newly synthesized DNA strand? How does Neisseria gonorrhoeae use slipped-strand mispairing to its advantage?

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Quiz 10: Molecular Regulation