What technique was used to show DNA molecules are replicated simultaneously at several sites along their length?
A)scanning electron microscopy
B)confocal laser scanning microscopy
C)autoradiographic analysis
D)polyacrylamide gel electrophoresis
E)isoelectric focusing

Question

Quizplus · Accepted Answer

Autoradiographic analysis involves labeling the DNA with radioactive nucleotides and visualizing the distribution of the radioactivity. In experiments demonstrating simultaneous replication, the DNA is labeled with two different radioactive isotopes, and autoradiographs are taken at different points during replication. If replication occurs simultaneously at multiple sites, the autoradiographs will show multiple bands corresponding to the different regions of the chromosome.

What Technique Was Used to Show DNA Molecules Are Replicated