Question 1

A transcription factor that binds to a gene first and facilitates the binding of other transcription factors is called a__________ .

Accepted Answer

The answer of A transcription factor that binds to a...

Question 2

Eukaryotes use these highly specialized enhancer elements, which regulate the transcription of multiple genes packaged in complexes of closely related genes (e.g., fi- globin).

Accepted Answer

The answer of Eukaryotes use these highly specialized enhancer elements,...

Question 3

Which protein complex directs DNA bending into loops that contact RNA polymerase and transcription factors bound at the core promoter or with protein complexes bound to proximal promoter elements?

Accepted Answer

The answer of Which protein complex directs DNA bending into...

Question 4

What effect does methylation of CpG islands have on human promoters?

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The answer of What effect does methylation of CpG islands...

Question 5

Enzymes that create epigenetic marks are sometimes referred to as writers, whereas enzymes that eliminate epigenetic marks are called_________ .

Accepted Answer

The answer of Enzymes that create epigenetic marks are sometimes...

Question 6

Which molecules bind regulatory sequences of DNA to encourage positive regulation of transcription?

Accepted Answer

The answer of Which molecules bind regulatory sequences of DNA...

Question 7

Gal80 is mutated such that it cannot interact with Gal3. What effect would you expect to see in the presence of galactose?

Accepted Answer

The answer of Gal80 is mutated such that it cannot...

Question 8

In the GAL gene system, which protein binds to the activation domain of the activator protein, ultimately blocking transcription in the absence of galactose?

Accepted Answer

The answer of In the GAL gene system, which protein...

Question 9

RNA polymerase can bind to _______ to generate different mRNAs from the same gene.

Accepted Answer

The answer of RNA polymerase can bind to _______ to...

Question 10

Many types of cancer are known to overexpress the receptor protein tyrosine kinase. Which molecular technique can be used to reduce expression of tyrosine kinase in cell culture?

Accepted Answer

RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Thus, it can be used to reduce the expression of tyrosine kinase in cell culture by targeting its mRNA.

Question 11

For the following gene, which mutant likely has lost its enhancer? $$\begin{array} { l c c } 
\text { Mutant \# } & \text { Deletion Region } & \text { \% Transcription } \
\text { WT } & \text { None } & 100 \% \
1 & 1 - 200 & 130 \% \
2 & 250 - 400 & 100 \% \
3 & 500 - 800 & 50 \% \
4 & 950 - 1100 & 0 \%
\end{array}$$

Accepted Answer

The answer of For the following gene, which mutant likely...

Question 12

What are the protein- binding sequences that direct enhancers to interact with the intended promoter and that block communication between enhancers and other promoters?

Accepted Answer

The answer of What are the protein- binding sequences that...

Question 13

Inherited traits that can change without affecting the sequence of DNA are examples of what?

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The answer of Inherited traits that can change without affecting...

Question 14

What are three proteins or groups of proteins you would expect to find bound to the core promoter region in eukaryotes?

Accepted Answer

The answer of What are three proteins or groups of...

Question 15

What are the three mechanisms by which chromatin remodelers can move nucleosomes?

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The answer of What are the three mechanisms by which...

Question 16

If you block histone deacetylase, what effect would you expect to see on transcriptional activity?

Accepted Answer

The answer of If you block histone deacetylase, what effect...

Question 17

In the GAL gene system, which protein acts as an activator protein through its transcription- initiating effect?

Accepted Answer

The answer of In the GAL gene system, which protein...

Question 18

Gal4 is mutated such that it still binds to the DNA but cannot interact with Gal80. What effect would you expect to see in the absence of galactose?

Accepted Answer

The answer of Gal4 is mutated such that it still...

Question 19

For the following gene, you notice the following results. $$\begin{array} { l c c c } 
\text { Mutant \# } & \text { Deletion Region } & \text { \% Transription (lungs) } & \text { \% Transaription (kid) } \
\hline \text { WT } & \text { None } & 100 \% & 100 \% \
1 & 1 - 200 & 130 \% & 130 \% \
2 & 250 - 400 & 100 \% & 100 \% \
3 & 500 - 800 & 50 \% & 50 \% \
4 & 950 - 1100 & 0 \% & 100 \%
\end{array}$$
 What type of sequence has been mutated in mutant 4?

Accepted Answer

The answer of For the following gene, you notice the...

Question 20

During RNAi, what do miRNAs target for destruction?

Accepted Answer

miRNAs (microRNAs) target mRNAs (messenger RNAs) for destruction or repression, thereby regulating gene expression post-transcriptionally.

Quiz 13: Regulation of Gene Expression in Bacteria and Bacteriophage

A transcription factor that binds to a gene first and facilitates the binding of other transcription factors is called a__________ .

Eukaryotes use these highly specialized enhancer elements, which regulate the transcription of multiple genes packaged in complexes of closely related genes (e.g., fi- globin).

Which protein complex directs DNA bending into loops that contact RNA polymerase and transcription factors bound at the core promoter or with protein complexes bound to proximal promoter elements?

What effect does methylation of CpG islands have on human promoters?

Enzymes that create epigenetic marks are sometimes referred to as writers, whereas enzymes that eliminate epigenetic marks are called_________ .

Which molecules bind regulatory sequences of DNA to encourage positive regulation of transcription?

Gal80 is mutated such that it cannot interact with Gal3. What effect would you expect to see in the presence of galactose?

In the GAL gene system, which protein binds to the activation domain of the activator protein, ultimately blocking transcription in the absence of galactose?

RNA polymerase can bind to _______ to generate different mRNAs from the same gene.

Many types of cancer are known to overexpress the receptor protein tyrosine kinase. Which molecular technique can be used to reduce expression of tyrosine kinase in cell culture?

What are the protein- binding sequences that direct enhancers to interact with the intended promoter and that block communication between enhancers and other promoters?

Inherited traits that can change without affecting the sequence of DNA are examples of what?

What are three proteins or groups of proteins you would expect to find bound to the core promoter region in eukaryotes?

What are the three mechanisms by which chromatin remodelers can move nucleosomes?

If you block histone deacetylase, what effect would you expect to see on transcriptional activity?

In the GAL gene system, which protein acts as an activator protein through its transcription- initiating effect?

Gal4 is mutated such that it still binds to the DNA but cannot interact with Gal80. What effect would you expect to see in the absence of galactose?

During RNAi, what do miRNAs target for destruction?