The following steps must be performed to make a bacterium produce human protein X. 1- Translation
2- Restriction enzyme
3- Prokaryotic transcription 4-DNA ligase
5- Transformation
6- Eukaryotic transcription 7-Reverse transcription
Which of the following places the steps in the correct order?
A) 6, 7, 2, 3, 4, 5, 1
B) 6, 2, 1, 3, 4, 5, 7
C) 1, 2, 3, 5, 4, 7, 6
D) 6, 7, 2, 4, 5, 3, 1
E) 5, 2, 3, 4, 7, 6, 1

Question

Quizplus · Accepted Answer

To produce human protein X in bacteria, start with eukaryotic transcription (6) to obtain mRNA, then reverse transcription (7) to convert mRNA to cDNA. Use restriction enzymes (2) to cut DNA, ligate (4) the cDNA into a plasmid, transform (5) the plasmid into bacteria, perform prokaryotic transcription (3), and finally translation (1) to produce the protein.

The Following Steps Must Be Performed to Make a Bacterium