Which of the following places the steps in the PCR procedure in the correct order?
1) Incubate at 94°C to denature DNA strands;
2) Incubate at 72°C for DNA synthesis;
3) Incubate at 60°C for primer hybridization.
A) 2; 1; 3
B) 1, 2, 3
C) 3, 2, 1
D) 1, 3, 2
E) 3; 1; 2

Question

Quizplus · Accepted Answer

The correct order of steps in PCR is: denaturation at 94°C, primer annealing at 60°C, and DNA synthesis at 72°C.

Which of the Following Places the Steps in the PCR