The final step in a Sanger DNA sequencing reaction is to run the DNA fragments on a gel. What purpose does this serve?
A) It adds ddNTP to the end of each DNA fragment.
B) It changes the length of the DNA fragments.
C) It separates DNA fragments based on their charge.
D) It separates DNA fragments generated during the sequencing reaction based on one-nucleotide differences in their size.

Question

Quizplus · Accepted Answer

Running the DNA fragments on a gel separates the DNA fragments generated during the sequencing reaction based on one-nucleotide differences in their size. This allows for identification of the individual nucleotides in the DNA sequence.

The Final Step in a Sanger DNA Sequencing Reaction Is