What information is critical to the success of polymerase chain reaction (PCR) itself?
A) The DNA sequence of the ends of the DNA to be amplified must be known.
B) The complete DNA sequence of the DNA to be amplified must be known.
C) The sequence of restriction-enzyme recognition sites in the DNA to be amplified must be known.
D) The sequence of restriction-enzyme recognition sites in the DNA to be amplified and in the plasmid where the amplified DNA fragment will be cloned must be known.

Question

Quizplus · Accepted Answer

Knowing the DNA sequence of the ends of the DNA to be amplified is critical for designing the PCR primers, which are essential for the amplification reaction. The other options are not necessary for the success of PCR itself, although they may be important for downstream applications or cloning purposes.

What Information Is Critical to the Success of Polymerase Chain