Which of the following processes would allow the detection of alternative splicing of transcripts from a given gene?
A) Compare the DNA sequence of the given gene to that of a similar gene in a related organism.
B) Measure the relative rates of transcription of the given gene compared to that of a gene known to be constitutively spliced.
C) Compare the sequences of different primary transcripts made from the given gene.
D) Compare the sequences of different mRNAs made from the given gene.

Question

Quizplus · Accepted Answer

To detect alternative splicing, we need to compare the sequences of different mRNA molecules produced from the same gene. Only choice D involves comparing different mRNA sequences. Choice A involves comparing DNA sequences, which may not reveal alternative splicing events. Choice B compares rates of transcription, which may not directly relate to alternative splicing. Choice C compares primary transcript sequences, which may not reflect the spliced mRNA sequence.

Which of the Following Processes Would Allow the Detection of Alternative