Essentials of Genetics Study Set 1
Quiz 13 :
Translation and Proteins
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Q02 Q02 Q02
A short segment of an mRNA molecule is shown below. The polypeptide it codes for is also shown: 5' -AUGGUGCUGAAG : methionine -valine -leucine -lysine Assume that a mutation in the DNA occurs so that the fourth base (counting from the 5' end) of the messenger RNA now reads A rather than G. What sequence of amino acids will the mRNA now code for? (You do not need a copy of the genetic code to answer the question.)
Q16 Q16 Q16
The following drawing represents simultaneous transcription and translation in E. coli. Answer the questions below the drawing. The arrow gives the direction of the RNA polymerase. a() Is the letter A nearer the 5' or the 3' end of the molecule? b() Is the letter B nearer the 5' or the 3' end of the molecule? c() Is the letter C nearer the 5' or the 3' end of the tRNA molecule? d() What is the "S" value for the large rRNA that is closest to the letter D? e() Which terminus (N or C) of the growing polypeptide chain is nearer to the letter E?
Q18 Q18 Q18
Three major types of RNAs are mRNA, rRNA, and tRNA. For each of the conditions below, predict the consequences in terms of the population of proteins being synthesized in a particular cell. What qualitative and quantitative changes, if any, are expected in the individual protein involved (if one is involved) and in the population of proteins in this cell? a() a frameshift mutation in the heterozygous state b() a deletion of a significant proportion of ribosomal genes (rDNA)
Q22 Q22 Q22
The following table presents the effect of different media on the growth response of tryptophan mutations in Salmonella typhimurium (+ = growth, - = no growth). a() Construct the biochemical pathway for the compounds IGP, AA, IN, and TRY based on these data. b() Place strains of bacteria (mutations) in the appropriate steps in the pathway. c() In bacteria, it is often possible to make partial diploid strains. Assume that a diploid strain was made containing the complete genomes of the trp -2 and trp -1 strains. Would this diploid strain be able to grow on the unsupplemented medium? Explain your answer.