Clinical Laboratory Immunology
Quiz 13 :
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Q14 Q14 Q14
Review the PCR protocol herein and identify its name. A single- stranded probe with internal complementary sequences folds on itself into a "hairpin" shape bringing a fluorescent molecule at one end of the strand in proximity to the quencher molecule at the other end of the strand. After PCR product is formed, during the next denaturation step the probe becomes single- stranded, binds to PCR target DNA, and separates the fluorescent molecule from the quencher molecule. PCR product is detected as increased fluorescence.
Q19 Q19 Q19
Review the protocol herein and identify its name. Two labeled probes attached to target DNA. DNA polymerase fills in the nucleotides between the p and DNA ligase joins the two pieces. The product is denatured into single strands, and the process i repeated. Amplified target DNA is detected via the labels on the probes.