Quiz 4: The Microscope

-Identify the microscope part labeled by the letter "d." A)Stage B)Base C)Arm D)Head

-Identify the microscope part labeled by the letter "k." A)Coarse adjustment knob B)Substage light C)Fine adjustment knob D)Iris diaphragm lever

-Identify the microscope part labeled by the letter "c." A)Ocular lens B)Objective lens C)Revolving nosepiece D)Condenser

What part of the microscope moves the slide on the stage? A)Fine adjustment knob B)Mechanical stage control C)Iris diaphragm D)Aperture

Which part of the microscope is the circular area on the stage that light passes through? A)Aperture B)Arm C)Iris diaphragm D)Ocular lens

On what part of the microscope are the objectives lenses located? A)Body tube B)Stage C)Base D)Rotating nosepiece

If you wanted to view a structure at the largest total magnification possible on your compound light microscope (1000× TM), which objective lens would you choose? A)Low-power lens B)Scanning lens C)Oil-immersion lens D)High-power lens

What part of the microscope regulates the amount of light that passes through the slide? A)Fine adjustment knob B)Condenser C)Substage light D)Iris diaphragm

The working distance is the ________. A)space between the slide and specimen and the objective lens B)space between the ocular and objective lens C)space between the condenser and ocular lens D)space between your eye and the ocular lens

Convert 4 mm into μm. A)0)04 μm B)0)004 μm C)4000 μm D)400 μm

Convert 62.3 μm into mm. A)0)623 mm B)62300 mm C)6230 mm D)0)0623 mm

An object extends halfway across the field of view. The field diameter for the oil-immersion objective is 0.2 mm. Estimate the size of the object in micrometers (μm). A)0)01 μm B)10 μm C)100 μm D)0)1 μm

If you have three threads laying on top of each other on a slide and you are focused on the top-most thread, then ________. A)all three threads should be in clear focus B)you are ready to rotate the ocular lens C)neither the middle nor the bottom-most thread will be in clear focus D)the middle thread will also be in clear focus

What is depth of field? A)Distance between the ocular and objective lenses B)Distance between the slide and the objective lens C)Another term for field diameter D)Thickness of a specimen when in focus

When you put away a microscope, which objective lens should be closest to the stage? A)Low-power B)Scanning C)Oil-immersion D)High-power

When transporting the microscope one hand should support the ________ and the other should support the ________. A)condenser; stage B)head; base C)arm; base D)stage; arm

When transporting a microscope, ________. A)the 100× objective lens should be closest to the stage B)the condenser should be removed and stored in its compartment C)use both hands to hold it by the base D)the cord should be secured

-The "l" marks the ________. A)fine adjustment knob B)light control C)coarse adjustment knob D)substage light

-The "b" marks the ________. A)objective lens B)revolving nosepiece C)arm D)ocular lens

-The "f" marks the ________. A)condenser B)substage light C)iris diaphragm D)revolving nosepiece

What part of a microscope anchors the objective lens to the head of the microscope? A)Base B)Revolving nosepiece C)Arm D)Stage

Which statement best describes what a letter viewed under the microscope would look like? A)Same as with the naked eye B)Sideways and right to left C)Upside down and backward D)Upside down, but still left to right

How would you calculate total magnification? A)Divide the ocular lens magnification by the objective lens magnification B)Multiply magnification of the ocular lens times that of the objective lens C)Add together the ocular and objective magnifications D)Divide the objective lens magnification by the ocular lens magnification

What is the total magnification when using the oil-immersion objective to view a structure? A)400× B)40× C)1000× D)100×

What is the working distance? A)Distance between the stage and light source B)Distance between the slide and structure and the objective lens C)Distance between the objective and ocular lenses D)Distance between the human eye and ocular lens

As total magnification increases, the working distance ________. A)stays the same B)decreases C)increases

Convert 245 μm into mm. A)245,000 mm B)0)245 mm C)0)0245 mm D)2)45 mm

Convert 0.75 mm into μm. A)0)75 μm B)750 μm C)0)0075 μm D)75 μm

The field diameter for the low-powered objective is ________ the field diameter for the scanning objective lens. A)less than B)equal to C)greater than

If the field diameter of the high-powered objective is 0.5 mm, what is the field diameter of this objective in µm? A)500 μm B)0)005 μm C)50 μm D)0)05 μm

The field diameter for the 4× objective on a specific microscope is 4.0 mm. What will the field diameter be for the 10× objective? A)2 mm B)1)6 mm C)10 mm D)40 mm

You are viewing a gland at 100× total magnification and the microscope at that magnification has a field diameter of 5 mm. The gland's width takes up one-quarter of the view. What is the size of the gland in mm? A)10 mm B)1)25 mm C)20 mm D)0)8 mm

What is the depth of field? A)The thickness of the specimen that is in sharp focus B)How easily focused the specimen is between two different magnifications C)The length of the specimen when viewed with the scanning objective lens D)How wide the view is through the lens

If you decrease the total magnification, the depth of the field ________. A)decreases B)stays the same C)increases

You should use lens paper and ________ to the clean the lenses. A)lens cleaner B)disinfectant C)water D)oil

You should allow the microscope to cool before storing it because ________. A)its motor and light are warm and a student could get injured B)it is easier to clean when cool C)it will extend the life of the expensive bulb D)it will prevent the motor from burning out

Compare regulating the amount of light with condensing the light. A)Condensers narrow the beam of light but do not change the amount of light. B)Both can be changed by using the iris diaphragm. C)Both can be used to concentrate the light passing through the specimen. D)You do not need to condense the light if you use the iris diaphragm correctly.

Which objective lens should be used to examine the entire wall of an organ from the exterior to the lumen? A)Scanning objective B)Low power C)High power D)Oil-immersion

Explain why the field diameter decreases with increasing magnification. A)The lens is closer to the specimen and less of the specimen can be illuminated. B)Increasing magnification decreases the working distance rather than the field diameter. C)Using the oil-immersion lens limits the ability to illuminate a specimen at high power. D)Parfocal microscopes gain the ability to stay in focus when changing objective lenses.

Calculate the total magnification of a specimen when using a standard ocular lens and a scanning objective lens. A)40X B)4X C)100X D)400X

What variables would you need to calculate the cross-sectional area of a blood vessel? A)Field diameter B)Total magnification C)Both field diameter and total magnification D)Neither field diameter nor total magnification