In the current form of dideoxy DNA sequencing, the primer is unlabeled and each dideoxynucleotide is labeled with a different fluorescent marker. This method
A) eliminates the need to run sequencing reaction products on a gel.
B) eliminates the need for sophisticated machines to read the output of each sequencing reaction.
C) allows a DNA sequence to be determined from 4 separate sequencing reactions, each run on a separate lane of a gel.
D) allows a DNA sequence to be determined from one sequencing reaction that is run on a single lane of a gel.

Question

Quizplus · Accepted Answer

This method allows for all four dideoxynucleotides to be included in a single reaction because each is labeled with a different fluorescent marker. This means the sequencing can be done in one reaction and analyzed in a single lane of a gel, with the sequence read by detecting the different colors of the fluorescent markers.

In the Current Form of Dideoxy DNA Sequencing, the Primer