When constructing a cDNA library from a particular organism, the choice of tissue is , whereas when constructing a genomic DNA library from the same organism, the choice of tissue is .
A) immaterial; immaterial
B) critical; immaterial
C) critical; critical
D) immaterial; critical

Question

Quizplus · Accepted Answer

When constructing a cDNA library, the choice of tissue is critical because cDNA libraries are made from mRNA, which varies significantly between different tissues due to differential gene expression. However, for a genomic DNA library, the choice of tissue is immaterial because the genomic DNA is the same in almost all cells of an organism.

When Constructing a CDNA Library from a Particular Organism, the Choice