Using the information provided,calculate the sperm concentration and sperm count:
-Using a 1:10 dilution of a specimen with a volume of 2 mL,400 sperm are counted in four large squares (WBC)of the Neubauer hemocytometer.
A) 5 million/mL and 10 million/specimen
B) 10 million/mL and 20 million/specimen
C) 30 million/mL and 60 million/specimen
D) 40 million/mL and 80 million/specimen

Question

Quizplus · Accepted Answer

The sperm concentration is calculated as (400 sperm / 4 squares) * 10^6 * dilution factor / number of squares counted = (100 * 10^6 * 10) / 4 = 10 million/mL. The total sperm count for the specimen is concentration * volume = 10 million/mL * 2 mL = 20 million/specimen.

Using the Information Provided,calculate the Sperm Concentration and Sperm Count