After DNA unwinds and becomes single-stranded in a PCR reaction, the temperature is lowered to allow the ____ to hybridize with the DNA template.
A) vectors
B) primers
C) tracers
D) restriction enzymes
E) DNA ligase

Question

After DNA unwinds and becomes single-stranded in a PCR reaction, the temperature is lowered to allow the ____ to hybridize with the DNA template.​
A) vectors​
B) ​primers
C) ​tracers
D) ​restriction enzymes
E) ​DNA ligase

Quizplus · Accepted Answer

Primers are short sequences of nucleotides that provide a starting point for DNA synthesis. In PCR, after the DNA is denatured, the temperature is lowered to allow these primers to anneal or hybridize to specific sequences on the single-stranded DNA template, enabling the DNA polymerase to initiate replication.

After DNA Unwinds and Becomes Single-Stranded in a PCR Reaction