Generating a phylogeny that represents the traditional groupings of bacteria requires sequencing and comparing the entire genome of each organism and cannot rely on single-gene phylogenies generated from 16S rRNA datasets.

Question

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Single-gene phylogenies, especially those generated from 16S rRNA datasets, have been widely used and are considered a standard for inferring bacterial phylogeny and taxonomy, as 16S rRNA genes are highly conserved across different bacterial species.

Generating a Phylogeny That Represents the Traditional Groupings of Bacteria