If FRAP is performed on two membranes, one at 25ºC and the other at 40ºC, which one of the following results would most likely be observed?
A)Only the membrane at the higher temperature would recover fluorescence in the bleached area.
B)Only the membrane at the lower temperature would recover fluorescence in the bleached area.
C)Both membranes would recover fluorescence in the bleached area, but the membrane at the colder temperature would recover more rapidly.
D)Both membranes would recover fluorescence in the bleached area, but the membrane at the warmer temperature would recover more rapidly.
E)Both membranes would recover fluorescence in the bleached area at the same rate.

Question

Quizplus · Accepted Answer

In FRAP, fluorescence recovery occurs as fluorescent molecules from the surrounding, unbleached area diffuse into the bleached area. The rate of diffusion is directly related to temperature, with higher temperatures resulting in faster diffusion. Therefore, the membrane at the warmer temperature (40ºC) would recover fluorescence more rapidly than the membrane at the colder temperature (25ºC). However, both membranes would still recover fluorescence in the bleached area.

If FRAP Is Performed on Two Membranes, One at 25ºC