Transformation in a cloning experiment is ________.
A) ligating pieces of foreign DNA together
B) inserting DNA into bacteria cells
C) cutting DNA with restriction enzymes
D) using PCR to clone a gene
E) a technique for determining gene copy number in a genome

Question

Quizplus · Accepted Answer

Transformation refers specifically to the process of introducing foreign DNA into bacterial cells, typically through the use of heat shock or electroporation. Ligating pieces of foreign DNA together, cutting DNA with restriction enzymes, and using PCR to clone a gene are all important steps in the cloning process but do not specifically involve transformation. Determining gene copy number in a genome is a separate technique altogether.

Transformation in a Cloning Experiment Is ________