In a recombinant DNA experiment, which enzyme is used to join together DNA fragments by forming phosphodiester bonds between nucleotides? Recall that this same enzyme joins together Okazaki fragments on the lagging strand during DNA replication.
A) DNA primase
B) DNA polymerase
C) DNA helicase
D) DNA ligase
E) Reverse transcriptase

Question

Quizplus · Accepted Answer

DNA ligase is used to join together DNA fragments by forming phosphodiester bonds between nucleotides in a recombinant DNA experiment, as well as in the joining of Okazaki fragments during DNA replication.

In a Recombinant DNA Experiment, Which Enzyme Is Used to Join