Which enzyme-catalyzed reaction is LEAST sensitive to changes in the concentration of its reactants under physiological conditions?
A) phosphoglycerate kinase
B) GAP dehydrogenase
C) adenylate kinase
D) fructose 1-phosphate aldolase
E) phosphofructokinase-1

Question

Quizplus · Accepted Answer

Phosphofructokinase-1 (PFK-1) is the rate-limiting enzyme in glycolysis and is highly regulated by various factors, including the concentration of its substrates and products. However, under physiological conditions, it is more sensitive to allosteric regulators such as ATP, ADP, AMP, and fructose 2,6-bisphosphate than to changes in the concentration of its reactants. This makes it less sensitive to changes in reactant concentrations compared to the other enzymes listed, which directly catalyze reactions in a more straightforward manner influenced by substrate concentration.

Which Enzyme-Catalyzed Reaction Is LEAST Sensitive to Changes in the Concentration