Which statement is TRUE regarding topoisomerases during synthesis of nucleic acids by E. coli RNA polymerase?
A) DNA gyrase is required to relieve supercoils on the DNA exit side of the polymerase.
B) Since no strand cleavage occurs, supercoiling is not a factor in the action of RNA polymerase.
C) Positive supercoils are generated ahead of the transcription bubble.
D) Both the DNA and the RNA product will be supercoiled.
E) None of the statements is true.

Question

Quizplus · Accepted Answer

During transcription, positive supercoils are generated ahead of the transcription bubble. Topoisomerases, including DNA gyrase, are required to relieve the buildup of positive supercoiling to allow for continued transcription. Supercoiling is therefore a factor in the action of RNA polymerase. None of the other statements are true.

Which Statement Is TRUE Regarding Topoisomerases During Synthesis of Nucleic