You want to purify a specific soluble protein from a solution of lysed cells so that you can conduct biochemical tests of the protein's function. To best achieve this, which type of antibody would be MOST effective to use in your affinity purification?
A) monoclonal IgE
B) polyclonal IgE
C) monoclonal IgG
D) polyclonal IgG
E) monoclonal IgM

Question

Quizplus · Accepted Answer

Monoclonal IgG antibodies are most effective for affinity purification because they are highly specific to a single epitope on the target protein, ensuring that only the desired protein is purified from the mixture.

You Want to Purify a Specific Soluble Protein from a Solution