A double-reciprocal plot of 1/V₀ versus 1/[S] for an enzyme in the presence of increasing concentrations of an uncompetitive inhibitor will have lines corresponding to the differentinhibitor concentrations that are BEST described by which statement? A) The lines will intersect at the x-axis to the left of the y-axis. B) The lines will cross the y-axis and will be parallel to each other. C) The lines will intersect to the left of the y-axis but above the x-axis. D) The lines will intersect below the x-axis, to the right of the y-axis. E) The lines will be parallel to each other but will not cross the y-axis.

Question

Quizplus · Accepted Answer

In the presence of an uncompetitive inhibitor, the double-reciprocal (Lineweaver-Burk) plot will show lines that cross the y-axis and are parallel to each other. This is because uncompetitive inhibition affects both Km and Vmax proportionally, causing a shift in the plot without altering the slope.

A Double-Reciprocal Plot of 1/V0 Versus 1/[S] for an Enzyme

A Double-Reciprocal Plot of 1/V₀ Versus 1/[S] for an Enzyme