When performing PCR, what is the advantage to using a DNA polymerase isolated from a thermophilic organism?
A) The PCR reaction does not need to be cooled below the denaturation step.
B) The PCR reaction does not need primers to proceed.
C) The PCR reaction does not need to have fresh enzyme added after each cycle.
D) The PCR reaction does not need to have fresh dNTPs added after each cycle.
E) The PCR reaction does not need to have fresh template DNA added after each cycle.

Question

Quizplus · Accepted Answer

DNA polymerase from thermophilic organisms is stable at high temperatures, which means it does not denature during the PCR's denaturation step. Therefore, it remains active throughout the cycles without the need for fresh enzyme addition.

When Performing PCR, What Is the Advantage to Using a DNA