Which factor is required to accurately determine the relative amount of gene expression of two or more genes in an organism using qPCR?
A) a "no template" control
B) an oligonucleotide probe labeled with a fluorophore and a quenching molecule at its ends
C) use of reverse transcriptase to copy the RNA products into DNA
D) All of the answers are correct.
E) None of the answers is correct.

Question

Quizplus · Accepted Answer

All the options listed are essential components or steps in the process of quantifying gene expression using qPCR. A "no template" control ensures specificity and absence of contamination, an oligonucleotide probe with a fluorophore and quencher enables detection of the PCR product, and reverse transcriptase is necessary for converting RNA into DNA for the PCR process.

Which Factor Is Required to Accurately Determine the Relative Amount