If you wanted to create specific DNA sequence changes to a plasmid in the coding sequence for a protein you are researching, but there are no suitably located restriction sites nearby, which technique would you use?
A) error-prone PCR
B) site-directed mutagenesis
C) oligonucleotide-directed mutagenesis
D) exposing the plasmid DNA to UV light
E) exposing the cells containing the plasmid DNA to chemical mutagens

Question

Quizplus · Accepted Answer

Oligonucleotide-directed mutagenesis allows for specific changes to be made at any location in the DNA sequence without depending on the presence of restriction sites. It involves the synthesis of short, single-stranded DNA molecules called oligonucleotides that are complementary to the target DNA sequence but contain the desired mutation. These oligonucleotides are then introduced to the plasmid-containing cells and used as a template for DNA polymerase-mediated incorporation of the mutations into the plasmid DNA.

If You Wanted to Create Specific DNA Sequence Changes to a Plasmid