Which statement regarding Type I and Type II restriction endonucleases is NOT accurate?
A) Type I endonucleases cleave DNA at random sites.
B) Type II endonucleases recognize small (4-6 bp) palindromic sequences.
C) Type II endonucleases always make staggered cuts in DNA strands.
D) Type I endonucleases require ATP.
E) Type I endonucleases are generally large, multisubunit complexes.

Question

Quizplus · Accepted Answer

Type II endonucleases may make either blunt-end or staggered cuts, depending on the specific enzyme and the position of the recognition sequence. Therefore, statement C is not always accurate.

Which Statement Regarding Type I and Type II Restriction Endonucleases