In the construction of a YAC library, each chromosome has the advantage of being able to hold very long DNA sequences. However, besides selectable marker(s)the YAC vector must minimally include which of the following?
A)a bacterial origin (ori)
B)cos site(s)and origin
C)centromere, telomeres, and yeast origin
D)centromere and origin
E)centromere and telomeres

Question

Quizplus · Accepted Answer

In addition to selectable marker(s), the YAC vector must minimally include centromere, telomeres, and yeast origin. The centromere is necessary for proper segregation during cell division, telomeres protect the ends of the DNA from degradation, and the yeast origin is required for replication within the yeast cell. The bacterial origin (ori) and cos site(s) are not necessary for YAC construction, as YAC cloning relies solely on the properties of yeast cells.

In the Construction of a YAC Library, Each Chromosome Has