Which of the following modifications is most likely to alter the rate at which a DNA fragment moves through a gel during electrophoresis?
A)altering the nucleotide sequence of the DNA fragment without adding or removing nucleotides
B)radioactively labeling the cytosine bases within the DNA fragment
C)increasing the length of the DNA fragment
D)leaving the length of the DNA fragment the same

Question

Quizplus · Accepted Answer

Increasing the length of the DNA fragment will alter its size and therefore its rate of movement through the gel. The larger the fragment, the slower it will move through the gel. Altering the nucleotide sequence without changing the size or radioactively labeling specific bases will not affect the rate of movement. Leaving the length of the fragment the same will also result in the same rate of movement as before.

Which of the Following Modifications Is Most Likely to Alter