At a specific area of a chromosome, the following sequence of nucleotides is present where the chain opens to form a replication fork:
3' C C T A G G C T G C A A T C C 5'
An RNA primer is formed starting at the underlined T (T) of the template. Which of the following represents the primer sequence?
A) 5' G C C T A G G 3'
B) 3' G C C T A G G 5'
C) 5' A C G T T A G G 3'
D) 5' A C G U U A G G 3'
E) 5' G C C U A G G 3'

Question

Quizplus · Accepted Answer

RNA primers are synthesized using the DNA template strand, and they are complementary and antiparallel to the template. The sequence starting from the underlined T in the template is 5' AGGCT... 3'. The complementary RNA sequence would be 5' UCCGA... 3', but since the primer starts at the T, the sequence is 5' UUAGG 3', which matches option D.

At a Specific Area of a Chromosome, the Following Sequence