What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes.
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V.Use ligase to seal plasmid DNA to nonplasmid DNA.
A) I, II, IV, III, V
B) II, III, V, IV, I
C) III, II, IV, V, I
D) III, IV, V, I, II
E) IV, V, I, II, III

Question

Quizplus · Accepted Answer

First, we need to extract the plasmid DNA from bacterial cells (III) that we will later modify. Then, we cut the plasmid DNA using restriction enzymes (II) to create sticky ends that will allow us to hydrogen-bond the plasmid DNA to the nonplasmid DNA fragments (IV). Next, we use ligase to seal the plasmid DNA to the nonplasmid DNA (V), creating a recombinant DNA molecule. Finally, we transform bacteria with the recombinant DNA molecule (I) to insert the plasmid into the bacterium. Therefore, the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium is III, II, IV, V, I.

What Is the Most Logical Sequence of Steps for Splicing