Why is a guide RNA needed when using CRISPR-Cas9 for gene editing?
A) to help Cas9 find the target DNA to cut
B) to help Cas9 be transported into the nucleus
C) to help repair the target DNA after it is cut
D) to help make copies of the target DNA

Question

Quizplus · Accepted Answer

The guide RNA helps the Cas9 nuclease locate the specific target DNA sequence to be cut, making it an essential component of the CRISPR-Cas9 gene editing system.

Why Is a Guide RNA Needed When Using CRISPR-Cas9 for Gene