When enzymes are purified,the assay is often based on
A) light absorbance.
B) catalytic activity.
C) pH.
D) temperature changes.
E) mRNA levels.

Question

Quizplus · Accepted Answer

Enzymes are known for their catalytic activity, hence when enzymes are purified, the most appropriate and widely used assay is based on the ability of the enzyme to catalyze a known chemical reaction. This assay is called an enzymatic assay and it measures the rate of the catalyzed reaction, which gives an indication of enzyme activity. Therefore, option B is the best choice. The other options, including light absorbance, pH, temperature changes, and mRNA levels are not as reliable and specific as enzymatic assays.

When Enzymes Are Purified,the Assay Is Often Based on