A mutation in the active site of succinyl CoA synthetase where His is converted to a lysine would result in which?
A) increased stable folding
B) the loss of a succinyl phosphate intermediate
C) the loss of a positive-charged amino acid necessary for catalysis
D) All of the above
E) None of the above

Question

Quizplus · Accepted Answer

The histidine in the active site of succinyl CoA synthetase plays a crucial role in the formation of the succinyl phosphate intermediate. Replacing it with lysine, which has different chemical properties, would likely disrupt this process, leading to the loss of the succinyl phosphate intermediate. Choices A and C are incorrect because lysine is also positively charged, and there's no direct evidence that this mutation would increase stable folding.

A Mutation in the Active Site of Succinyl CoA Synthetase