What is responsible for joining eukaryotic Okazaki fragments together?
A)DNA ligase
B)DNA polymerase $\beta$
C)DNA polymerase $\delta$
D)DNA polymerase $\varepsilon$
E)RNase H1

Question

What is responsible for joining eukaryotic Okazaki fragments together?
A)DNA ligase
B)DNA polymerase $\beta$
C)DNA polymerase  $\delta$
D)DNA polymerase $\varepsilon$
E)RNase H1

Quizplus · Accepted Answer

The enzyme responsible for joining eukaryotic Okazaki fragments together is DNA ligase. It creates a phosphodiester bond between the 3' hydroxyl group of one nucleotide and the 5' phosphate group of another nucleotide, ultimately sealing the nicks between adjacent Okazaki fragments. DNA polymerases are responsible for the synthesis of the fragments themselves, but cannot join them together. RNase H1 is a ribonuclease that cleaves RNA in RNA-DNA hybrid molecules, and is not involved in the joining of Okazaki fragments. Among the three DNA polymerases listed, only polymerase δ and ε are involved in DNA replication in eukaryotic cells while polymerase β is mainly involved in DNA repair, but none of them can join Okazaki fragments together.

What Is Responsible for Joining Eukaryotic Okazaki Fragments Together? A)DNA β\betaβ

What Is Responsible for Joining Eukaryotic Okazaki Fragments Together?
A)DNA $\beta$