Which of the following would not be a useful procedure for dissociating the subunits of a multimeric protein in order to sequence the individual subunits?
A) Exposure to pH extremes (ie, pH 1 or pH 13).
B) High salt concentrations.
C) 6 N HCl at 110°C for 24 hours.
D) 8 M urea.
E) 6 M guanidinium chloride.

Question

Quizplus · Accepted Answer

C) 6 N HCl at 110°C for 24 hours would not be useful for merely dissociating subunits for sequencing because it would hydrolyze peptide bonds, thus breaking down the protein into its constituent amino acids, rather than just dissociating the subunits.

Which of the Following Would Not Be a Useful Procedure