All are necessary for restriction endonuclease mapping of DNA molecules EXCEPT:
A) Determining the 5'- and/or 3'-ends of the molecule.
B) Electrophoresis of fragments.
C) Hydrolysis with two or more endonucleases.
D) Knowing where each endonuclease hydrolyzes.
E) All are true.

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All are necessary for restriction endonuclease mapping of DNA molecules.

All Are Necessary for Restriction Endonuclease Mapping of DNA Molecules