Which of the following statements correctly identifies a type II restriction endonuclease?
A) They work on both DNA and RNA.
B) They recognize a palindromic sequence and cut just before the palindromic sequence.
C) The result of this endonuclease is blunt ends.
D) They degrade DNA by subsequently removing bases from each end.
E) They cut DNA only at sites in specific nucleotide sequences with a two-fold axis of symmetry.

Question

Quizplus · Accepted Answer

Type II restriction endonucleases cut DNA only at sites in specific nucleotide sequences with a two-fold axis of symmetry. These sequences are often palindromic, meaning they are the same forward and backward. The result can be blunt ends, or overhangs depending on the enzyme. They do not work on RNA and do not degrade DNA by subsequently removing bases from each end.

Which of the Following Statements Correctly Identifies a Type II