For an enzyme-catalyzed reaction, the initial velocity was determined at two different concentrations of the substrate. Which of the following would be closest to the value of V max ?

A) 4.7 mM/min
B) 0.67 mM/min
C) 3.19 mM/min
D) 1.5 mM/min
E) 0.32 mM/min

Question

For an enzyme-catalyzed reaction, the initial velocity was determined at two different concentrations of the substrate. Which of the following would be closest to the value of V max ?

A) 4.7 mM/min 
B) 0.67 mM/min 
C) 3.19 mM/min 
D) 1.5 mM/min 
E) 0.32 mM/min

Quizplus · Accepted Answer

VS1U1B1max is the maximum initial velocity, which is achieved when all enzyme active sites are saturated with substrate. In this case, the substrate concentration closest to saturating the enzyme will be the higher concentration measured. Therefore, the closest value to VS1U1B1maxS1U1B0 can be calculated as the highest initial velocity measured, which is 3.19 mM/min at a substrate concentration of 8 mM.

For an Enzyme-Catalyzed Reaction, the Initial Velocity Was Determined at Two