The function of DNA ligase in the generation of recombinant DNA is to
A) cut DNA.
B) replicate DNA.
C) unwind DNA.
D) join DNA fragments by the formation of covalent bonds.
E) join DNA fragments noncovalently.

Question

Quizplus · Accepted Answer

DNA ligase is an enzyme responsible for the joining of DNA fragments. It does so by the formation of covalent bonds between the phosphate backbone of adjacent DNA strands, sealing any nicks or gaps in the sugar-phosphate backbone. This process is important in the generation of recombinant DNA by ligating together DNA fragments from different sources.

The Function of DNA Ligase in the Generation of Recombinant