Which of the following necessarily makes use of reverse transcriptase?
A) Southern blotting
B) Construction of a gene library
C) Construction of a cDNA library
D) Gene knockout studies
E) Pharming

Question

Quizplus · Accepted Answer

Construction of a cDNA library involves the conversion of mRNA to cDNA using reverse transcriptase, which makes it the only option that necessarily involves the use of this enzyme. Southern blotting involves the use of probes to detect DNA fragments, gene library construction typically involves the use of restriction enzymes and ligases to create recombinant DNA molecules, gene knockout studies involve targeted mutations of genes, and pharming involves the production of proteins using genetically modified organisms.

Which of the Following Necessarily Makes Use of Reverse Transcriptase