What is the major difference between using green fluorescent protein versus using an antibiotic resistance gene as a reporter gene?
A) The green fluorescent protein can be used only when the host cell is a plant cell because it relies on the process of photosynthesis.
B) The antibiotic resistance gene can be used only when the host cell is an animal.
C) The green fluorescent protein can be used only in transfection, not in transformation.
D) With the green fluorescent protein, no cells are killed in the selection process.
E) Homologous recombination will work only with the green fluorescent protein.

Question

Quizplus · Accepted Answer

The major difference between using green fluorescent protein and an antibiotic resistance gene as a reporter gene is that the selection process with green fluorescent protein does not kill cells, whereas the selection process with an antibiotic resistance gene involves killing cells that do not contain the gene. This allows for easier and more efficient screening of cells that have successfully incorporated the reporter gene.

What Is the Major Difference Between Using Green Fluorescent Protein