Recombinant DNA technology requires not only getting the DNA into the cell, but also getting it to replicate appropriately. In this process, the specificity of which of the following enzymes can present a challenge?
A) DNA ligase
B) DNA polymerase
C) RNA polymerase
D) Reverse transcriptase
E) Replicase

Question

Quizplus · Accepted Answer

DNA polymerase is the enzyme responsible for replicating DNA in a cell, and it must match the nucleotides it adds to the DNA strand with the complementary base already present. When introducing recombinant DNA, the DNA polymerase may not recognize or be able to accurately replicate the foreign DNA, causing errors or even preventing replication altogether. Therefore, finding a suitable DNA polymerase is crucial for successful recombinant DNA technology.

Recombinant DNA Technology Requires Not Only Getting the DNA into the Cell