The initial velocity condition in the derivation of the Michaelis-Menten expression
A) allows measurement of product formation near equilibrium.
B) may not be attainable if the equilibrium constant is very large in favor of product formation.
C) requires that measurements be made before product accumulates significantly.
D) requires that the enzyme-substrate complex be formed very slowly.
E) none of the above

Question

Quizplus · Accepted Answer

The initial velocity condition in the Michaelis-Menten derivation requires that measurements be made before product accumulates significantly to ensure that the reaction rate reflects only the formation of product from substrate, not the reverse reaction or product interference.

The Initial Velocity Condition in the Derivation of the Michaelis-Menten