In a two substrate-two product enzymatic reaction, the apparent Km for one of the substrates is
A) independent of the concentration of the other substrate.
B) dependent upon the concentration of the products.
C) dependent upon the concentration of the other substrate.
D) dependent upon the concentration of enzyme.
E) dependent upon the maximum velocity.

Question

Quizplus · Accepted Answer

The apparent Km for one of the substrates in a two substrate-two product enzymatic reaction is dependent upon the concentration of the other substrate. This is because the binding of one substrate affects the binding of the other substrate due to changes in the enzyme's conformation. As a result, the apparent affinity (Km) for one substrate can increase or decrease depending on the concentration of the other substrate. The concentration of enzyme, products, or maximum velocity does not affect the Km value for a substrate.

In a Two Substrate-Two Product Enzymatic Reaction, the Apparent Km