Polymerase chain reaction (PCR) results in discrete length products because
A) Taq polymerase can only synthesize DNA of discrete size from any DNA template.
B) the reaction is terminated by denaturation at the same time following each round of polymerization.
C) the primers are of discrete size.
D) of the exponential production of run-off products.
E) the template for amplification is always a specific cloned DNA fragment.

Question

Quizplus · Accepted Answer

PCR results in exponential amplification of the target sequence, leading to an accumulation of run-off products of different sizes. However, as the reaction progresses, the amount of amplification product of a specific size becomes dominant, resulting in discrete length products.

Polymerase Chain Reaction (PCR) Results in Discrete Length Products Because