In constructing a genomic DNA library, limited digestion with a restriction enzyme that recognizes and cuts a 4 base pair sequence
A) ensures that the DNA fragments being cloned are very small.
B) ensures that introns are separated from exons.
C) generates overlapping fragments of the genome.
D) separates repetitive sequences from unique sequences.

Question

Quizplus · Accepted Answer

Limited digestion with a restriction enzyme that recognizes and cuts a 4 base pair sequence generates overlapping fragments of the genome, which is crucial for ensuring comprehensive coverage and assembly of the genome in the library.

In Constructing a Genomic DNA Library, Limited Digestion with a Restriction