Segments of pure samples of identical, relatively short DNA molecules from plasmids can be subdivided into smaller pieces by
A) Southern blot analysis.
B) Colony hybridization
C) Restriction enzyme digestion.
D) Agarose gel electrophoresis.
E) Ethidium bromide treatment

Question

Quizplus · Accepted Answer

Restriction enzymes specifically cleave DNA at certain nucleotide sequences, creating smaller pieces that can be separated by agarose gel electrophoresis. Southern blot analysis and colony hybridization are techniques used to detect specific DNA fragments, but not to subdivide them. Ethidium bromide treatment is used to visualize DNA fragments in agarose gels, but it does not subdivide them.

Segments of Pure Samples of Identical, Relatively Short DNA Molecules