A technique that is used to determine the abundance of a particular mRNA is
A) gel shift assay.
B) dideoxy chain termination.
C) Southern blotting.
D) DNA ligation.
E) Northern blotting.

Question

Quizplus · Accepted Answer

Northern blotting is the technique used to determine the abundance of a particular mRNA. It involves separating the mRNAs on a gel and then transferring them onto a membrane. The membrane is then probed with a radioactively labeled DNA or RNA probe that is specific for the mRNA of interest. The amount of signal produced by the probe is indicative of the abundance of the mRNA in the original sample. Therefore, option E is the correct answer.

A Technique That Is Used to Determine the Abundance of a Particular